CJ32 (let-7 minigene)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17769||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepCaSpeR4-tubulin promoter
Backbone manufacturerCarl Thummel Lab
- Backbone size w/o insert (bp) 10200
Vector typeDrosophila P-element vector
Growth in Bacteria
Gene/Insert namedme-let-7 minigene
SpeciesD. melanogaster (fly)
Insert Size (bp)870
Entrez Genelet-7 (a.k.a. Dmel_CR32968, CR32968, Dmel\CR32968, Let-7, dme-let-7, let7)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
let-7 was amplified from genomic DNA with primers 474 bp upstream and 310 bp downstream of the let-7 hairpin. pCaSpeR4 contains a ~2.4 kb tubulin promoter inserted between the EcoRI and KpnI sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CJ32 (let-7 minigene) was a gift from David Bartel (Addgene plasmid # 17769 ; http://n2t.net/addgene:17769 ; RRID:Addgene_17769)
For your References section:Intronic microRNA precursors that bypass Drosha processing. Ruby JG, Jan CH, Bartel DP. Nature. 2007 Jul 5. 448(7149):83-6. 10.1038/nature05983 PubMed 17589500