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pDL12c
(Plasmid #17835)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17835 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAST30
  • Backbone size w/o insert (bp) 3088
  • Vector type
    Synthetic Biology ; Aspergillus nidulans expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gpdA promoter, lacZ, argB_1626,
  • Alt name
    nutritional selection marker
  • Alt name
    β-galactosidase monomer
  • Species
    A. nidulans, E. coli
  • Mutation
    The argB_1626 mutation consists of two sequential stop codons at 925 bp downstream of the start codon
  • GenBank ID
    U24705

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Fungal Genetics Stock Center of University of Kansas
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDL12c was a gift from Jay Keasling (Addgene plasmid # 17835 ; http://n2t.net/addgene:17835 ; RRID:Addgene_17835)
  • For your References section:

    Marker and promoter effects on heterologous expression in Aspergillus nidulans. Lubertozzi D, Keasling JD. Appl Microbiol Biotechnol. 2006 Oct . 72(5):1014-23. 10.1007/s00253-006-0368-8 PubMed 16699756