pEGFP-N1-jGCaMP7b-XC
(Plasmid
#178361)
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PurposeImproved genetic encoded calcium sensor based on GCaMP7b.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 178361 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEGFP-N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namejGCaMP7b-XC
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site n/a (unknown if destroyed)
- 3′ cloning site n/a (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.01.09.475579v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-N1-jGCaMP7b-XC was a gift from Xiaodong Liu (Addgene plasmid # 178361 ; http://n2t.net/addgene:178361 ; RRID:Addgene_178361) -
For your References section:
Chronic Ca(2+) imaging of cortical neurons with long-term expression of GCaMP-X. Geng J, Tang Y, Yu Z, Gao Y, Li W, Lu Y, Wang B, Zhou H, Li P, Liu N, Wang P, Fan Y, Yang Y, Guo ZV, Liu X. Elife. 2022 Oct 5;11:e76691. doi: 10.7554/eLife.76691. 10.7554/eLife.76691 PubMed 36196992
