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GCN5 core-dCas9-p300 core
(Plasmid #179553)


Item Catalog # Description Quantity Price (USD)
Plasmid 179553 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    LentiCRISPR v2
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
    S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    D10A; H840A in S.pyogenes Cas9
  • Entrez Gene
    EP300 (a.k.a. KAT3B, MKHK2, RSTS2, p300)
  • Entrez Gene
    KAT2A (a.k.a. GCN5, GCN5L2, PCAF-b, hGCN5)
  • Promoter EF-1alpha
  • Tag / Fusion Protein
    • Flag Tag (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GCN5 core-dCas9-p300 core was a gift from Isaac Hilton (Addgene plasmid # 179553 ; ; RRID:Addgene_179553)
  • For your References section:

    Systematic comparison of CRISPR-based transcriptional activators uncovers gene-regulatory features of enhancer-promoter interactions. Wang K, Escobar M, Li J, Mahata B, Goell J, Shah S, Cluck M, Hilton IB. Nucleic Acids Res. 2022 Aug 12;50(14):7842-7855. doi: 10.1093/nar/gkac582. 10.1093/nar/gkac582 PubMed 35849129