Systematic comparison of CRISPR-based transcriptional activators uncovers gene-regulatory features of enhancer-promoter interactions.
Wang K, Escobar M, Li J, Mahata B, Goell J, Shah S, Cluck M, Hilton IB
Nucleic Acids Res. 2022 Aug 12;50(14):7842-7855. doi: 10.1093/nar/gkac582. PubMed Article
Wang K, Escobar M, Li J, Mahata B, Goell J, Shah S, Cluck M, Hilton IB
Nucleic Acids Res. 2022 Aug 12;50(14):7842-7855. doi: 10.1093/nar/gkac582. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 179543 | dCas9-CBP core | encodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter |
| 179544 | dCas9-p300 HAT | encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) driven by AF4 promoter |
| 179545 | dCas9-p300 PHD-HAT | encodes S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) driven by EF-1alpha promoter |
| 179546 | dCas9-p300 RING-PHD-HAT | encodes S. pyogenes dCas9 with c-terminal fusion of human p300 RING-PHD-HAT domain (aa 1155-1664) driven by EF-1alpha promoter |
| 179547 | dCas9-CBP HAT | encodes S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) driven by EF-1alpha promoter |
| 179548 | dCas9-CBP PHD-HAT | encodes S. pyogenes dCas9 with c-terminal fusion of human CBP PHD-HAT domain (aa 1279-1701) driven by EF-1alpha promoter |
| 179549 | dCas9-CBP RING-PHD-HAT | encodes S. pyogenes dCas9 with c-terminal fusion of human CBP RING-PHD-HAT domain (aa 1191-1701) driven by EF-1alpha promoter |
| 179550 | dCas9-CBP FL | encodes S. pyogenes dCas9 with c-terminal fusion of full length human CBP driven by EF-1alpha promoter |
| 179551 | dCas9-GCN5 core | encodes S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) driven by EF-1alpha promoter |
| 179552 | dCas9-GCN5 FL | encodes S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 driven by EF-1alpha promoter |
| 179553 | GCN5 core-dCas9-p300 core | encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter |
| 179554 | GCN5 FL-dCas9-p300 core | encodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter |
| 179555 | GCN5 core-dCas9-CBP core | encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter |
| 179556 | GCN5 FL-dCas9-CBP core | encodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter |
| 179557 | p300 core-dCas9-p300 core | encodes S. pyogenes dCas9 with both n-terminal and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter |
| 179558 | CBP core-dCas9-p300 core | encodes S. pyogenes dCas9 with n-terminal fusion of human CBP core (aa 1084-1701) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter |
| 179559 | p300 core-dCas9-CBP core | encodes S. pyogenes dCas9 with n-terminal fusion of human p300 core (aa 1048-1664) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter |
| 179560 | CBP core-dCas9-CBP core | encodes S. pyogenes dCas9 with both n-terminal and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter |