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PurposeTripartite split-GFP mammalian. Express GFP1-9 detector fragment
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 182244 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepCMV
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Backbone manufacturerpEGFP (Clontech)
- Total vector size (bp) 4614
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP 1-9 OPT
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SpeciesSynthetic
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Insert Size (bp)585
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer CMV-Rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV GFP1-9 was a gift from Stéphanie Cabantous (Addgene plasmid # 182244 ; http://n2t.net/addgene:182244 ; RRID:Addgene_182244) -
For your References section:
A new protein-protein interaction sensor based on tripartite split-GFP association. Cabantous S, Nguyen HB, Pedelacq JD, Koraichi F, Chaudhary A, Ganguly K, Lockard MA, Favre G, Terwilliger TC, Waldo GS. Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038/srep02854. 10.1038/srep02854 PubMed 24092409
Map uploaded by the depositor.
