pSEM416 - mosTI unc-119 P_UPN - gfp(nls) - tbb-2
(Plasmid #182359)

• Purpose: Positive control for mosTI(unc-119). unc-119 rescue and pan-neuronal nuclear GFP expression after mosTI insertion.
• Depositing Lab: Christian Froekjaer-Jensen
• Publication: El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068. doi: 10.1093/g3journal/jkac184.

Backbone

• Vector backbone: pTwist-Amp-high-copy
• Backbone manufacturer: Twist Biosciences
• Total vector size (bp): 5932
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ultra-pan-neuronal positive control pUNP::gfp(NLS)
• Species: C. elegans (nematode)
• Promoter: Promoter Ultra Pan Neuronal

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (destroyed during cloning)
• 3′ cloning site: BsaI (destroyed during cloning)
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • pSEM416 - mosTI unc-119 P_UPN - gfp(nls) - tbb-2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

www.wormbuilder.org
MOdular Safe harbor Transgene Insertion (mosTI).
Positive control vector: P_UPN::gfp(NLS)::tbb-2 UTR
Will give unc-119 rescue and neuronal nuclear GFP expression after insertion into any unc-119 mosTI sites.
See Yemini et al. 2021 Cell for development and characterization of the ultra-pan-neuronal promoter.

Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v1.full for bioRxiv preprint.