Lentiviral mBmal1-Luc
(Plasmid #182762)

• Purpose: Lentiviral vector encoding the promoter region of mouse Bmal1 gene driving a luciferase reporter
• Depositing Lab: Qing-Jun Meng
• Publication: Gossan et al Arthritis Rheum. 2013 Sep;65(9):2334-45. doi: 10.1002/art.38035.

Backbone

• Vector backbone: Lentiviral transfer vector
• Backbone size w/o insert (bp): 7400
• Total vector size (bp): 8049
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mouse arntl promoter
• Species: M. musculus (mouse)
• Insert Size (bp): 649
• Entrez Gene: Bmal1 (a.k.a. Arnt3, Arntl, BMAL1b, MOP3, bHLHe5, bmal1b')
• Promoter: 649 bp

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde I (not destroyed)
• 3′ cloning site: Sal I (not destroyed)
• 5′ sequencing primer: GAGGGATGGGCGAAGAGAT
• 3′ sequencing primer: GATCCCGCGGCGGCGGCG

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry