pSBbi G418 Hu Her2
(Plasmid #183246)

• Purpose: Expression of human HER2 antigen in a Sleeping Beauty transposon vector
• Depositing Lab: Alexander McLellan
• Publication: Rad S M et al PLoS One. 2020 Jul 24;15(7):e0232915. doi: 10.1371/journal.pone.0232915. eCollection 2020.

Backbone

• Vector backbone: pSBbi-Bla
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Her2
• Species: H. sapiens (human)
• Entrez Gene: ERBB2 (a.k.a. CD340, HER-2, HER-2/neu, HER2, MLN 19, MLN-19, NEU, NGL, TKR1, VSCN2, c-ERB-2, c-ERB2, p185(erbB2))

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pSBbi-G418R HuHer2Ag.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Useful for construction of artificial antigen presenting cells. See PMID: 37585342 for an additional reference.