pCMV_HaloK73T_cyto
(Plasmid
#183687)
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PurposeMammalian expression of cytosolic metastable Halotag/folding sensor (Cyto HaloK73T)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 183687 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCyto Halo(K73T)
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SpeciesBacterial
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MutationK73T in Halotag
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV_HaloK73T_cyto was a gift from Edward Avezov (Addgene plasmid # 183687 ; http://n2t.net/addgene:183687 ; RRID:Addgene_183687) -
For your References section:
Stress-induced protein disaggregation in the endoplasmic reticulum catalysed by BiP. Melo EP, Konno T, Farace I, Awadelkareem MA, Skov LR, Teodoro F, Sancho TP, Paton AW, Paton JC, Fares M, Paulo PMR, Zhang X, Avezov E. Nat Commun. 2022 May 6;13(1):2501. doi: 10.1038/s41467-022-30238-2. 10.1038/s41467-022-30238-2 PubMed 35523806
