PurposeExpression of human SOX2 and GFP under the human GFAP ABC1D promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||183909||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Not published but this plasmid was based on the following publication:
In vivo reprogramming of astrocytes to neuroblasts in the adult brain. Niu W, Zang T, Zou Y, Fang S, Smith DK, Bachoo R, Zhang CL. Nat Cell Biol. 2013 Oct;15(10):1164-75. doi: 10.1038/ncb2843. Epub 2013 Sep 22. https://pubmed.ncbi.nlm.nih.gov/24056302/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LV-hGFAP-SOX2-IRES-GFP was a gift from Chun-Li Zhang (Addgene plasmid # 183909 ; http://n2t.net/addgene:183909 ; RRID:Addgene_183909)