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(Plasmid #183953)


Item Catalog # Description Quantity Price (USD)
Plasmid 183953 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2278
  • Total vector size (bp) 4830
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    SARS-CoV-2 minigenome with Nano luciferase
  • Species
    SARS coronavirus 2
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    N (a.k.a. GU280_gp10)
  • Entrez Gene
    ORF10 (a.k.a. GU280_gp11)
  • Promoter Mammalian Pol I

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer GTGAGCGCGACGTAATAC
  • 3′ sequencing primer GCTGGCACGACAGGTTTC
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Invitrogen/GeneArt: The synthetic gene pPolI_SARS2_NLuc was assembled from synthetic oligonucleotides and/or PCR products by Invitrogen/GeneArt

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPolI_SARS-CoV-2_NLuc was a gift from AJ te Velthuis (Addgene plasmid # 183953 ; ; RRID:Addgene_183953)
  • For your References section:

    A SARS-CoV-2 mini-genome assay based on negative-sense RNA to study replication inhibitors and emerging mutations. Vial TK, Oade MS, Russell CA, Eggink D, te Velthuis AJW. bioRxiv 2021.06.28.450211 10.1101/2021.06.28.450211