|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||183953||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2278
- Total vector size (bp) 4830
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
- Cloning method Unknown
- 5′ sequencing primer GTGAGCGCGACGTAATAC
- 3′ sequencing primer GCTGGCACGACAGGTTTC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please visit https://www.biorxiv.org/content/10.1101/2021.06.28.450211v2 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pPolI_SARS-CoV-2_NLuc was a gift from AJ te Velthuis (Addgene plasmid # 183953 ; http://n2t.net/addgene:183953 ; RRID:Addgene_183953)
For your References section:A SARS-CoV-2 mini-genome assay based on negative-sense RNA to study replication inhibitors and emerging mutations. Vial TK, Oade MS, Russell CA, Eggink D, te Velthuis AJW. bioRxiv 2021.06.28.450211 10.1101/2021.06.28.450211