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PurposeEncodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter
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Depositing Lab
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Depositing OrganizationPrinceton University
Located in United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 183953 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 183953-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 183953-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepMK-RQ
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2278
- Total vector size (bp) 4830
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer GTGAGCGCGACGTAATAC
- 3′ sequencing primer GCTGGCACGACAGGTTTC
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byInvitrogen/GeneArt: The synthetic gene pPolI_SARS2_NLuc was assembled from synthetic oligonucleotides and/or PCR products by Invitrogen/GeneArt
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.06.28.450211v2 for bioRxiv preprint.
DNA (Catalog # 183953-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 183953-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPolI_SARS-CoV-2_NLuc was a gift from AJ te Velthuis (Addgene plasmid # 183953 ; http://n2t.net/addgene:183953 ; RRID:Addgene_183953) -
For your References section:
A SARS-CoV-2 mini-genome assay based on negative-sense RNA to study replication inhibitors and emerging mutations. Vial TK, Oade MS, Russell CA, Eggink D, te Velthuis AJW. bioRxiv 2021.06.28.450211 10.1101/2021.06.28.450211
Map uploaded by the depositor.
Map uploaded by the depositor.
