pT2iD6Hoxa13bER4(#686)
(Plasmid #184101)

• Purpose: cyclofen-inducible fish Hoxa13b activation via mammalian cell transfection or mRNA synthesis
• Depositing Lab: Sophie Vriz
• Publication: Vriz lab cyclofen-inducible ERT2 plasmids (unpublished)

Backbone

• Vector backbone: pT2iD6 (pCS2 derivative)
• Backbone manufacturer: M Volovitch
• Backbone size w/o insert (bp): 3794
• Vector type: Mammalian Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ZfHoxa13b-ERT2
• Species: D. rerio (ZfHoxa13b), H. sapiens (ERT2)
• Insert Size (bp): 1890
• Mutation: ERT2: G400V, M543A, L544A, deleted 1-281;
• Promoter: sCMV+SP6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SnaBI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: SVLrev34

Resource Information

• A portion of this plasmid was derived from a plasmid made by: synthetic ERT2

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

Depositor Comments

ZfHoxa13b: NP_571269; ERT2: NP_000116;