FLAG-SENP2m
(Plasmid #18713)

• Depositing Lab: Edward Yeh
• Publication: Kang et al Mol Cell. 2010 Apr 23. 38(2):191-201.

Backbone

• Vector backbone: PFLAG-CMV
• Backbone manufacturer: Sigma
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SENP2
• Species: H. sapiens (human)
• Insert Size (bp): 1770
• Mutation: R577L, K578M
• Entrez Gene: SENP2 (a.k.a. AXAM2, SMT3IP2)
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry