pWZL hygro 12S E1A
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18748||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepWZL Hygro
Backbone manufacturerAvailable at Addgene (#18750)
- Backbone size w/o insert (bp) 5915
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name12S E1A
Alt nameE1A 12S
Alt nameE1a protein from 12s mrna
Insert Size (bp)701
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer pBABE 5'
- 3′ sequencing primer IRES-R (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pWZL hygro 12S E1A was a gift from Scott Lowe (Addgene plasmid # 18748 ; http://n2t.net/addgene:18748 ; RRID:Addgene_18748)
For your References section:Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a. Serrano M, Lin AW, McCurrach ME, Beach D, Lowe SW. Cell. 1997 Mar 7. 88(5):593-602. 10.1016/S0092-8674(00)81902-9 PubMed 9054499