pLenti-Paxillin EGFP
(Plasmid
#187688)
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PurposeIn vivo visualization of Paxillin
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 187688 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLenti6.3/V5-DEST
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePaxillin
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SpeciesG. gallus (chicken)
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Insert Size (bp)1677
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MutationWT
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Entrez GenePXN (a.k.a. TGFB1I1)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-Paxillin EGFP was a gift from Mary Beckerle (Addgene plasmid # 187688 ; http://n2t.net/addgene:187688 ; RRID:Addgene_187688) -
For your References section:
LIM domains target actin regulators paxillin and zyxin to sites of stress fiber strain. Smith MA, Blankman E, Deakin NO, Hoffman LM, Jensen CC, Turner CE, Beckerle MC. PLoS One. 2013 Aug 21;8(8):e69378. doi: 10.1371/journal.pone.0069378. eCollection 2013. 10.1371/journal.pone.0069378 PubMed 23990882
