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(Plasmid #187901)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187901 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 6197
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    Triple alanine mutant: P228A, E230A, P232A
  • GenBank ID
  • Entrez Gene
    SQSTM1 (a.k.a. A170, DMRV, EBIAP, FTDALS3, NADGP, OSIL, PDB3, ZIP3, p60, p62, p62B)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BstGI (not destroyed)
  • 3′ cloning site XmaI (not destroyed)
  • 5′ sequencing primer pd2.5-mCherry 5'-CACCTACAAGGCCAAGAAGC
  • 3′ sequencing primer pEGFP-C Rev2 5'-CTTTATTTGTAACCATTATAAGCTGC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    S. Martens (University of Vienna); Wurzer et al., eLife 2015

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-p62-TIR-AAA was a gift from Erika Holzbaur (Addgene plasmid # 187901 ; ; RRID:Addgene_187901)
  • For your References section:

    Damaged mitochondria recruit the effector NEMO to activate NF-kappaB signaling. Harding O, Holzer E, Riley JF, Martens S, Holzbaur ELF. Mol Cell. 2023 Sep 7;83(17):3188-3204.e7. doi: 10.1016/j.molcel.2023.08.005. 10.1016/j.molcel.2023.08.005 PubMed 37683611