|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18863||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Gene/Insert nameStromal Interaction Molecule 2
SpeciesH. sapiens (human)
MutationCFP inserted between signal peptide and residues 15-746 of STIM2 (Lab plasmid WS5170)
/ Fusion Protein
- CFP (N terminal on backbone)
- Cloning method Gateway Cloning
- 5′ cloning site attB1 (unknown if destroyed)
- 3′ cloning site attB2 (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
STIM2 also has P38L, V222I, and E575V, as described in the paper's supplemental material. These mutations do not affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEX-CMV-SP-CFP-STM2(15-746) was a gift from Tobias Meyer (Addgene plasmid # 18863 ; http://n2t.net/addgene:18863 ; RRID:Addgene_18863)
For your References section:STIM2 is a feedback regulator that stabilizes basal cytosolic and endoplasmic reticulum Ca2+ levels. Brandman O, Liou J, Park WS, Meyer T. Cell. 2007 Dec 28. 131(7):1327-39. 10.1016/j.cell.2007.11.039 PubMed 18160041