|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||189552||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepSCM167.2-Venus (Fu lab)
Backbone manufacturerCustomized backbone from Fu lab
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameADAM metallopeptidase domain 10
Alt nameADAM10; RAK; kuz; AD10; AD18; MADM; CD156c; CDw156; HsT18717
SpeciesH. sapiens (human)
Entrez GeneADAM10 (a.k.a. AD10, AD18, CD156c, CDw156, HsT18717, MADM, RAK, kuz)
/ Fusion Protein
- VF (N terminal on insert)
- Cloning method Gateway Cloning
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note: Plasmid contains L60S, D96G, S115G, Y316H, and S700G mutations in hADAM10 compared to the NCBI reference sequence NP_001101.1. These mutations were present in the original DNA that was was PCR amplified from human placenta cDNA and are not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSCM167.2-Venus-VF-hADAM10 was a gift from Haian Fu (Addgene plasmid # 189552 ; http://n2t.net/addgene:189552 ; RRID:Addgene_189552)