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Addgene

pLD-puro-Cc-SARS-CoV-2_Spike_Lambda-VA
(Plasmid #191220)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191220 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLD-puro-CcVA (Addgene plasmid #24588)
  • Backbone size w/o insert (bp) 7737
  • Total vector size (bp) 11535
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SPIKE_SARS2_Lambda
  • Alt name
    S_Lambda
  • Species
    SARS CoV-2
  • Insert Size (bp)
    3798
  • Mutation
    G75V T76I Del 246-252 L452Q F490S D614G T859N
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Promoter CMV
  • Tag / Fusion Protein
    • VA tag (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hPGK_R [5'-ccttggaaaaggcgcaacc-3']
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLD-puro-Cc-SARS-CoV-2_Spike_Lambda-VA was a gift from Mohan Babu (Addgene plasmid # 191220 ; http://n2t.net/addgene:191220 ; RRID:Addgene_191220)
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