E. coli BL21 (DE3) ΔEntD
(Bacterial strain #192874)

• Purpose: Deletion of native E. coli EntD, a 4'-PPTase required for native enterobactin synthesis. Allows expression of NRPS carrier proteins that do not harbor a phosphopantetheinyl group.
• Depositing Labs: Christian Chalut, Christophe Guilhot
• Publication: Chalut et al Proc Natl Acad Sci U S A. 2006 May 30;103(22):8511-6. doi: 10.1073/pnas.0511129103. Epub 2006 May 18.

Backbone

• Vector backbone: n/a
• Vector type: This is a strain, not a plasmid

Growth in Bacteria

• Bacterial Resistance(s): None
• Growth Temperature: 37°C
• Growth Strain(s): E. coli BL21 (DE3) ΔEntD
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: The strain has a 535 nt deletion between nt 15 and nt 551 in the EntD gene.
• Species: E.coli

Cloning Information

• Cloning method: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

EntD, a 4'-phosphopantetheinyl transferase, is required for native enterobactin synthesis. This strain allows for expression of nonribosomal peptide synthetase (NRPS) carrier proteins that do not harbor a phosphopantetheinyl group.