pMV361_MjTyrRS
(Plasmid #193250)

• Purpose: Expresses the M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS)
• Depositing Labs: Peter Schultz, Jessica Seeliger
• Publication: Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348. doi: 10.1021/acsinfecdis.6b00179. Epub 2017 Mar 21.

Backbone

• Vector backbone: pMV361
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MjTyrRS
• Species: M. jannaschii
• Promoter: hsp60

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: cGGTGACCACAACGCG

Resource Information

• Supplemental Documents:
  • Previti_B7F_2_pMV361-BpaRS_pLann.gbk
• A portion of this plasmid was derived from a plasmid made by: Subcloned from pEVOL-pBpF (Addgene # 31190)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid was originally reported by the Peter Schultz lab in Wang, Schultz et al. PLoS One 2010, doi: 10.1371/journal.pone.0009354. Please see associated publication Ganapathy, Seeliger et al. for full details regarding any additional cloning that was performed.