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pSMT-MjtRNA-GFP(47TAG)
(Plasmid #193251)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 193251 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSMT3
  • Vector type
    Bacterial Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    GFP(47TAG)
  • Alt name
    GFP(151TAG), GFP(Tyr151TAG), eGFP
  • Mutation
    Codon for Tyr47 replaced with amber stop codon TAG (nucleotide position 151).
  • Promoter hsp60

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    MjtRNA
  • Alt name
    tRNATyr
  • Species
    M. jannaschii
  • Promoter metU

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid was originally reported by the Peter Schultz lab in Wang, Schultz et al. PLoS One 2010, doi: 10.1371/journal.pone.0009354. Please see associated publication Ganapathy, Seeliger et al. for full details regarding any additional cloning that was performed.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSMT-MjtRNA-GFP(47TAG) was a gift from Peter Schultz & Jessica Seeliger (Addgene plasmid # 193251 ; http://n2t.net/addgene:193251 ; RRID:Addgene_193251)
  • For your References section:

    A Screen for Protein-Protein Interactions in Live Mycobacteria Reveals a Functional Link between the Virulence-Associated Lipid Transporter LprG and the Mycolyltransferase Antigen 85A. Touchette MH, Van Vlack ER, Bai L, Kim J, Cognetta AB 3rd, Previti ML, Backus KM, Martin DW, Cravatt BF, Seeliger JC. ACS Infect Dis. 2017 May 12;3(5):336-348. doi: 10.1021/acsinfecdis.6b00179. Epub 2017 Mar 21. 10.1021/acsinfecdis.6b00179 PubMed 28276676