vc241
(Plasmid #193453)

• Purpose: Expression of rRFP in A. castellanii
• Depositing Lab: Chantal Abergel
• Publication: Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi: 10.1038/s41467-023-36145-4.

Backbone

• Vector backbone: vc2
• Backbone size w/o insert (bp): 675
• Total vector size (bp): 5580
• Modifications to backbone: Geneticin selection cassette has been repleaced for a nourseothricin selection cassette
• Vector type: Acanthamoeba exression
• Selectable markers: nourseothricin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mRFP
• Species: Acanthamoeba castellanii
• Insert Size (bp): 675
• Promoter: GAPDH

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: gccCATATGGCCTCCTCCGAGGACGTC
• 3′ sequencing primer: gcctctagattaGGCGCCGGTGGAGTG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry