PurposeExpresses human alpha-Synuclein A53T
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||194242||Standard format: Plasmid sent in bacteria as agar stab||1||$85 *|
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- Backbone size w/o insert (bp) 6180
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)473
Entrez GeneSNCA (a.k.a. NACP, PARK1, PARK4, PD1)
- Promoter SAR-CAG
/ Fusion Protein
- HA (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
Please note: The plasmid contains an 11bp deletion in one of the ITRs. This deletion does not affect plasmid performance as the virus undergoes self-repair of this region during AAV packaging.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAM/SAR-CAG-aSyn(A53T)/HA-WPRE-bGHpA was a gift from Michael J Fox Foundation MJFF (Addgene plasmid # 194242 ; http://n2t.net/addgene:194242 ; RRID:Addgene_194242)