pAM/SAR-CAG-aSyn(A53T)/HA-WPRE-bGHpA
(Plasmid #194242)

• Purpose: Expresses human alpha-Synuclein A53T
• Depositing Lab: Michael J Fox Foundation MJFF
• Publication: GeneDetect Michael J Fox Foundation Shared Tools Plasmids 2022 (unpublished)

Backbone

• Vector backbone: pAM
• Backbone size w/o insert (bp): 6180
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: aSynuclein(A53T)
• Species: H. sapiens (human)
• Insert Size (bp): 473
• Mutation: A53T
• Entrez Gene: SNCA (a.k.a. NACP, PARK1, PARK4, PD1)
• Promoter: SAR-CAG
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Please note: The plasmid contains an 11bp deletion in one of the ITRs. This deletion does not affect plasmid performance as the virus undergoes self-repair of this region during AAV packaging.