pscAAV-CBh-tagBFP2-WPRE3-enSV40pA
(Plasmid #194246)

• Purpose: Expresses tagBFP2
• Depositing Lab: Michael J Fox Foundation MJFF
• Publication: GeneDetect Michael J Fox Foundation Shared Tools Plasmids 2022 (unpublished)

Backbone

• Vector backbone: pscAAV
• Backbone size w/o insert (bp): 4257
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: tagBFP2
• Species: Synthetic
• Insert Size (bp): 746
• Promoter: CBh

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (unknown if destroyed)
• 3′ cloning site: StuI (unknown if destroyed)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Vladislav Verkhusha: Addgene #34632 (BAD-mTagBFP2)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Industry MTA
  • Evrogen Ancillary Agreement for FP Distribution to Industry

Depositor Comments

Please note: Plasmid contains a 22bp deletion in the Left ITR scAAV. This deletion is not known to affect plasmid function as the AAV will repair the ITRs itself upon packaging as long as one ITR is intact. The plasmid packaged fine and give high viral titers for the depositing lab.