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GST-14-3-3dm (pEBG)
(Plasmid #1943)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 1943 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    14-3-3 zeta
  • Alt name
  • Alt name
  • Species
    M. musculus (mouse)
  • Mutation
    Dimerization-deficient mutant of 14-3-3zeta aa: E5K, L12AE to Q12QR, Y82Q, K85N, and E87Q
  • Entrez Gene
    Ywhaz (a.k.a. 1110013I11Rik, 14-3-3zeta, AI596267, AL022924, AU020854)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRV (unknown if destroyed)
  • 5′ sequencing primer pGEX-5'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-14-3-3dm (pEBG) was a gift from Joseph Avruch (Addgene plasmid # 1943 ; ; RRID:Addgene_1943)
  • For your References section:

    A dimeric 14-3-3 protein is an essential cofactor for Raf kinase activity. Tzivion G, Luo Z, Avruch J. Nature 1998 Jul 2;394(6688):88-92. 10.1038/27938 PubMed 9665134