pcDNA216-CoV2-RBD-beta variant
(Plasmid
#194598)
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PurposeMammalian expression of RBD domain from SARS-CoV-2 beta (B.1.351)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 194598 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA216
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCoV2-RBD-beta variant
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SpeciesSevere acute respiratory syndrome coronavirus 2
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Entrez GeneS (a.k.a. GU280_gp02, spike glycoprotein)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA216-CoV2-RBD-beta variant was a gift from Ben Murrell (Addgene plasmid # 194598 ; http://n2t.net/addgene:194598 ; RRID:Addgene_194598) -
For your References section:
Beta RBD boost broadens antibody-mediated protection against SARS-CoV-2 variants in animal models. Sheward DJ, Mandolesi M, Urgard E, Kim C, Hanke L, Perez Vidakovics L, Pankow A, Smith NL, Castro Dopico X, McInerney GM, Coquet JM, Karlsson Hedestam GB, Murrell B. Cell Rep Med. 2021 Nov 16;2(11):100450. doi: 10.1016/j.xcrm.2021.100450. Epub 2021 Oct 23. 10.1016/j.xcrm.2021.100450 PubMed 34723224
