huntingtin_FL_NbioCflag
(Plasmid #194767)

• Purpose: Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells
• Depositing Lab: Cheryl Arrowsmith
• Depositing Organization: University of Toronto
  Located in Canada
• Publication: Unpublished huntingtin plasmids (unpublished)

Backbone

• Vector backbone: pBacMam2-DiEx-LIC
• Total vector size (bp): 16182
• Vector type: Mammalian Expression ; Baculovirus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HTT
• Alt name: huntingtin
• Alt name: huntingtin:TOC031-E08:C247750
• Species: H. sapiens (human)
• Mutation: Q23 (polyQ repeat)
• Entrez Gene: HTT (a.k.a. HD, IT15, LOMARS)
• Promoter: CMV and P10
• Tags / Fusion Proteins:
  • Avi-tag (biotin acceptor peptide) (N terminal on insert)
  • Flag-tag (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: pFBM-fwd (5'-caaaatgtcgtaacaactccgc-3')
• 3′ sequencing primer: pFBM-rev (5'- tagttaagaataccagtcaatctttcac-3')

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Kazusa DNA Research Institute Gene KIEE4296 & Original Clone ID cp94296

DNA (Catalog # 194767-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 194767-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.