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(Plasmid #194999)


Item Catalog # Description Quantity Price (USD)
Plasmid 194999 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    SARS-CoV-2 protein S-protein receptor binding domain
  • Species
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Promoter T7lac
  • Tag / Fusion Protein
    • His8-Zbasic (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

LIC cloning used 5' site BsaI (destroyed in cloning) and 3' site HindIII (not destroyed).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pExp-His-zBasic-RBD was a gift from Marko Hyvönen (Addgene plasmid # 194999 ; ; RRID:Addgene_194999)
  • For your References section:

    Multivalent bicyclic peptides are an effective antiviral modality that can potently inhibit SARS-CoV-2. Gaynor KU, Vaysburd M, Harman MAJ, Albecka A, Jeffrey P, Beswick P, Papa G, Chen L, Mallery D, McGuinness B, Van Rietschoten K, Stanway S, Brear P, Lulla A, Ciazynska K, Chang VT, Sharp J, Neary M, Box H, Herriott J, Kijak E, Tatham L, Bentley EG, Sharma P, Kirby A, Han X, Stewart JP, Owen A, Briggs JAG, Hyvonen M, Skynner MJ, James LC. Nat Commun. 2023 Jun 16;14(1):3583. doi: 10.1038/s41467-023-39158-1. 10.1038/s41467-023-39158-1 PubMed 37328472