MK01
(Bacterial strain #195090)

• Purpose: Improved E. coli strain created by knocking out the endogenous lacI gene with chloramphenicol acetyltransferase (cat) selection cassette in the parent strain BW27783 that overcome the all-or-none response of araBAD promoter (PBAD).
• Depositing Lab: Sander Tans
• Publication: Kogenaru et al J Biol Eng. 2014 Jan 2;8(1):2. doi: 10.1186/1754-1611-8-2.

Backbone

• Vector backbone: n/a
• Vector type: This is a strain, not a plasmid

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 15-30 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): MK01
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Replaced lacI gene with chloramphenicol acetyltransferase (cat) selection cassette in the parent strain BW27783.
• Species: n/a

Cloning Information

• Cloning method: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

MK01 strain was derived by knocking out the endogenous lacI gene with chloramphenicol acetyltransferase (cat) selection cassette in the parent strain BW27783. The parent strain encodes araC gene but carries a deletion for the arabinose metabolizing genes, ∆(araH-araF). The all-or-none response of araBAD promoter (PBAD) response is overcome by inserting a copy of the low-affinity high-capacity transporter, araE, under the control of an arabinose-independent promoter.