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(Plasmid #19770)


Item Catalog # Description Quantity Price (USD)
Plasmid 19770 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 32900
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
  • Copy number
    Low Copy


  • Gene/Insert name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Entrez Gene
    Klf4 (a.k.a. EZF, Gklf, Zie)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MfeI (unknown if destroyed)
  • 3′ cloning site PacI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

From article: For the cloning of adenoviral vectors, EcoRI fragments containing the respective cDNAs
for c-myc (constitutively active human T58A variant), Klf4, Oct4 and Sox2 were
integrated into the EcoRI site of pHIHG-Ad2. A fragment containing the cDNA sequence
was isolated from the resulting plasmids by MfeI/PacI digestion and used for
electroporation of BJ5183 cells together with linearized vector pAd-ClaI. Correct clones
were identified by HindIII digestion.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAd-Klf4 was a gift from Konrad Hochedlinger (Addgene plasmid # 19770 ; ; RRID:Addgene_19770)
  • For your References section:

    Induced Pluripotent Stem Cells Generated Without Viral Integration. Stadtfeld M, Nagaya M, Utikal J, Weir G, Hochedlinger K. Science. 2008 Sep 25. ():. 10.1126/science.1162494 PubMed 18818365