p3806
(Plasmid
#198148)
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PurposeMammalian expression of LAMP1-miniSOG
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 198148 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePCMV::LAMP1::tdminiSOG::mCherry
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SpeciesH. sapiens (human)
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Entrez GeneLAMP1 (a.k.a. CD107a, LAMPA, LGP120)
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Tag
/ Fusion Protein
- tdminiSOG-mCherry (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3806 was a gift from Michael Hensel (Addgene plasmid # 198148 ; http://n2t.net/addgene:198148 ; RRID:Addgene_198148) -
For your References section:
Single molecule analyses reveal dynamics of Salmonella translocated effector proteins in host cell endomembranes. Goser V, Sander N, Schulte M, Scharte F, Franzkoch R, Liss V, Psathaki OE, Hensel M. Nat Commun. 2023 Mar 4;14(1):1240. doi: 10.1038/s41467-023-36758-9. 10.1038/s41467-023-36758-9 PubMed 36870997