pLenti-C-STAT1-mGFP-P2A-Puro
(Plasmid #199345)

• Purpose: express murine STAT1 protein fused with mGFP
• Depositing Lab: Massimo Mallardo
• Publication: Caiazza et al Int J Mol Sci. 2022 Nov 17;23(22):14232. doi: 10.3390/ijms232214232.

Backbone

• Vector backbone: pLenti-C-mGFP-P2A-Puro
• Backbone size w/o insert (bp): 7750
• Total vector size (bp): 9919
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: signal transducer and activator of transcription 1
• Alt name: STAT1
• Species: M. musculus (mouse)
• Insert Size (bp): 2247
• GenBank ID: NM_001205313.1
• Entrez Gene: Stat1 (a.k.a. 2010005J02Rik)
• Promoter: CMV
• Tag / Fusion Protein:
  • mGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry