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Addgene

pCBL101-RUBY
(Plasmid #199723)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199723 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCBL101
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    35S-RUBY
  • Species
    Synthetic
  • Insert Size (bp)
    4892

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene sequencing identified partial duplication of the CaMV 35S enhancer element. Depositing lab has confirmed that the duplication was present in their original stock of the plasmid and does not disrupt RUBY function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCBL101-RUBY was a gift from Kan Wang (Addgene plasmid # 199723 ; http://n2t.net/addgene:199723 ; RRID:Addgene_199723)

  • For your References section:

    New T-DNA binary vectors with NptII selection and RUBY reporter for efficient maize transformation and targeted mutagenesis. Lee K, Kang M, Ji Q, Grosic S, Wang K. Plant Physiol. 2023 Apr 18:kiad231. doi: 10.1093/plphys/kiad231. 10.1093/plphys/kiad231 PubMed 37070560
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