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pcDNA3 Split random
(Plasmid #200646)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200646 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3
  • Total vector size (bp) 6562
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Split GFP with chimeric intron containing random sequence
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1876
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 Split random was a gift from Claire Francastel (Addgene plasmid # 200646 ; http://n2t.net/addgene:200646 ; RRID:Addgene_200646)

  • For your References section:

    Small nucleolar RNAs promote the restoration of muscle differentiation defects in cells from myotonic dystrophy type 1. Bogard B, Bonnet H, Boyarchuk E, Tellier G, Furling D, Mouly V, Francastel C, Hube F. Nucleic Acids Res. 2025 Mar 20;53(6):gkaf232. doi: 10.1093/nar/gkaf232. 10.1093/nar/gkaf232 PubMed 40156865
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