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pBS-Squ:mCherry
(Plasmid #20163)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 20163 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    spaghetti squash (squ, myosin II RLC) 5' UTR and ORF fused to mCherry followed by squ 3' UTR
  • Alt name
    sqh spaghetti squash
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    3503
  • Entrez Gene
    sqh (a.k.a. Dmel_CG3595, CG3595, DmMRLC_C, Dmel\CG3595, MLC, MLRC, MRLC, Myo, Myo II, Myo-II, Myo-II RLC, MyoII, Myosin II, NMM, RLC, RLC2, RMLC, Sqh, actomyosin, myo II, myoII, myosin, myosin 2, myosin II, pMyo-II, sgh, spg, squ, ssq)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are several polymorphisms in the 3’UTR of the expression construct and should not affect the function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-Squ:mCherry was a gift from Eric Wieschaus (Addgene plasmid # 20163 ; http://n2t.net/addgene:20163 ; RRID:Addgene_20163)
  • For your References section:

    Pulsed contractions of an actin-myosin network drive apical constriction. Martin AC, Kaschube M, Wieschaus EF. Nature. 2008 Nov 23. ():. 10.1038/nature07522 PubMed 19029882