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(Plasmid #24287)

Full plasmid sequence is not available for this item.



Item Catalog # Description Quantity Price (USD)
Plasmid 24287 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 6786
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    dia (a.k.a. Dmel_CG1768, 38E.16, CG1768, DIA, Dia, Diaphanous, Dias, Dmel\CG1768, l(2)k07135, ms(2)04138)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AttR1 (unknown if destroyed)
  • 3′ cloning site AttR2 (unknown if destroyed)
  • 5′ sequencing primer M13 Fwd
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    I received the cDNA from the lab of Mark Peifer at UNC. I amplified the cDNA and inserted it into the pENTR D Topo vector and then ligated it into the pMT-WG vector using LR cloning.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT-Diaphanous-GFP was a gift from Ron Vale (Addgene plasmid # 24287 ; ; RRID:Addgene_24287)
  • For your References section:

    Dynamics of myosin, microtubules, and Kinesin-6 at the cortex during cytokinesis in Drosophila S2 cells. Vale RD, Spudich JA, Griffis ER. J Cell Biol. 2009 Sep 7. 186(5):727-38. 10.1083/jcb.200902083 PubMed 19720876