pCE059-SiT-ddCas12a(E993A)-3xNLS-VP64-triplex-DR-M13-DR-pA
(Plasmid
#202044)
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PurposeEncodes a DNase-dead AsCpf1 fused to a 3x SV40 nuclear localization signal (NLS) and VP64 transactivation domain. 3' UTR has a MALAT1 triplex, dCas12a direct repeats (DR), and a crRNA cloning site.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 202044 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepAAV-MCS
- Total vector size (bp) 9442
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameddCas12a(E993A)-3xNLS-VP64
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gRNA/shRNA sequenceM13
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.05.08.539911 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCE059-SiT-ddCas12a(E993A)-3xNLS-VP64-triplex-DR-M13-DR-pA was a gift from Darren Cusanovich (Addgene plasmid # 202044 ; http://n2t.net/addgene:202044 ; RRID:Addgene_202044) -
For your References section:
Optimization of a Cas12a-Driven Synthetic Gene Regulatory Network System. Kang H, Fitch JC, Varghese RP, Thorne CA, Cusanovich DA. ACS Synth Biol. 2025 May 16;14(5):1732-1744. doi: 10.1021/acssynbio.5c00084. Epub 2025 May 2. 10.1021/acssynbio.5c00084 PubMed 40316310
