pL0_CD-eCFP
              
              
                (Plasmid
                
                #202142)
              
            
            
            
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            PurposeLevel 0 plasmid containing an eCFP fluorescent protein with CD overhangs used to build a level 1 construct.
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              Depositing Lab
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          Sequence Information
 
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 202142 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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            Vector backbonepL0R
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              Vector typeSynthetic Biology
 
Growth in Bacteria
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            Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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            Growth Temperature37°C
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            Growth Strain(s)DH5alpha
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            Copy numberHigh Copy
 
Gene/Insert
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                Gene/Insert nameeCFP gene
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                    SpeciesAequorea victoria
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                  Insert Size (bp)724
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                  MutationNone
 
Cloning Information
- Cloning method Gibson Cloning
 
Resource Information
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            Supplemental Documents
 
Terms and Licenses
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        Academic/Nonprofit Terms
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      Industry Terms
- Not Available to Industry
 
 
Trademarks:
- Zeocin® is an InvivoGen trademark.
 
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pL0_CD-eCFP was a gift from Christopher Dupont (Addgene plasmid # 202142 ; http://n2t.net/addgene:202142 ; RRID:Addgene_202142) - 
                
For your References section:
Universal loop assembly: open, efficient and cross-kingdom DNA fabrication. Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, Bielinski V, von Dassow P, Dupont CL, Federici F. Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. Epub 2020 Feb 5. 10.1093/synbio/ysaa001 PubMed 32161816