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PP_070_pAAV_CAG_LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2
(Plasmid #203228)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203228 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 5827
  • Total vector size (bp) 9577
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Membrane-localized optopatch for neuronal dendrites
  • Species
    Synthetic
  • Insert Size (bp)
    3750
  • Promoter CAG

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGGGCCCGGGATCTCGAGgatccGCCACCATGGGCCGACCCCAAATACTC
  • 3′ sequencing primer tatcgataagcttgatatcgaattcTTAAACTTCATTTTCATAGCAAAATC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.06.02.543490 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PP_070_pAAV_CAG_LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2 was a gift from Adam Cohen (Addgene plasmid # 203228 ; http://n2t.net/addgene:203228 ; RRID:Addgene_203228)

  • For your References section:

    Dendritic excitations govern back-propagation via a spike-rate accelerometer. Park P, Wong-Campos JD, Itkis DG, Lee BH, Qi Y, Davis HC, Antin B, Pasarkar A, Grimm JB, Plutkis SE, Holland KL, Paninski L, Lavis LD, Cohen AE. Nat Commun. 2025 Feb 4;16(1):1333. doi: 10.1038/s41467-025-55819-9. 10.1038/s41467-025-55819-9 PubMed 39905023
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