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Addgene

UBQ10pro-nlsABACUS2-400n-NosT
(Plasmid #203728)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203728 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFR7m34GW
  • Backbone size w/o insert (bp) 10000
  • Total vector size (bp) 14986
  • Vector type
    Plant Expression, Synthetic Biology
  • Selectable markers
    Fast-RED

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    nlsABACUS2-400n
  • Species
    A. thaliana (mustard weed), Synthetic
  • Insert Size (bp)
    2394
  • Promoter AtUBQ10pro 1500bp
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UBQ10pro-nlsABACUS2-400n-NosT was a gift from Alexander Jones (Addgene plasmid # 203728 ; http://n2t.net/addgene:203728 ; RRID:Addgene_203728)

  • For your References section:

    Next-generation ABACUS biosensors reveal cellular ABA dynamics driving root growth at low aerial humidity. Rowe J, Grange-Guermente M, Exposito-Rodriguez M, Wimalasekera R, Lenz MO, Shetty KN, Cutler SR, Jones AM. Nat Plants. 2023 Jun 26. doi: 10.1038/s41477-023-01447-4. 10.1038/s41477-023-01447-4 PubMed 37365314
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