TCARZ-CBh-GFP-SV40PA-GF3-R4A22
(Plasmid #203767)

• Purpose: For production of template RNA for use in PRINT, GFP under CBh promoter
• Depositing Lab: Kathleen Collins
• Publication: Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.1038/s41587-024-02137-y.

Backbone

• Vector backbone: pUC57min
• Vector type: IVT template

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PRINT template RNA for GFP
• Species: Synthetic
• Promoter: CBh

Cloning Information

• Cloning method: Unknown

Resource Information

• Supplemental Documents:
  • tcarz-cbh-gfp-sv40pa-gf3-r4a22.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Dimers and multimers have been observed to exist in our stock of this plasmid. Multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.