pEF-1α_ZF9LOV[C2]-VP64 (pLZA126)
(Plasmid
#203911)
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PurposeEncodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C2 position in mammalian cells
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Depositing Lab
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Depositing OrganizationPrinceton University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 203911 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 203911-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 203911-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepVITRO1-NEO-MCS
- Backbone size w/o insert (bp) 6994
- Total vector size (bp) 7897
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameZF9LOV[C2]-VP64
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SpeciesSynthetic
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Insert Size (bp)903
- Promoter EF1α
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer aaaccaccgctaattcaaagcaac
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 203911-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 203911-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF-1α_ZF9LOV[C2]-VP64 (pLZA126) was a gift from Jared Toettcher (Addgene plasmid # 203911 ; http://n2t.net/addgene:203911 ; RRID:Addgene_203911) -
For your References section:
Light-switchable transcription factors obtained by direct screening in mammalian cells. Zhu L, McNamara HM, Toettcher JE. Nat Commun. 2023 Jun 2;14(1):3185. doi: 10.1038/s41467-023-38993-6. 10.1038/s41467-023-38993-6 PubMed 37268649
Map uploaded by the depositor.
