pcDNA3.1_T6B -mCherry
(Plasmid
#205600)
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Purposeexpresses human T6B peptide fused with mChery in mammalian cells
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Depositing Lab
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Depositing OrganizationStockholms Universitet
Located in Sweden -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 205600 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 205600-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 205600-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameT6B peptide (fragment of human TRNC6B 599-683aa)
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Alt nameTRNC6B , KIAA1093
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SpeciesH. sapiens (human)
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GenBank IDTRNC6B (Homo sapiens)
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Entrez GeneTNRC6B (a.k.a. GDSBA)
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Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.08.10.503472v1 for bioRxiv preprint.
DNA (Catalog # 205600-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 205600-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1_T6B -mCherry was a gift from Marc Friedlander (Addgene plasmid # 205600 ; http://n2t.net/addgene:205600 ; RRID:Addgene_205600) -
For your References section:
Detection of transcriptome-wide microRNA-target interactions in single cells with agoTRIBE. Sekar V, Marmol-Sanchez E, Kalogeropoulos P, Stanicek L, Sagredo EA, Widmark A, Doukoumopoulos E, Bonath F, Biryukova I, Friedlander MR. Nat Biotechnol. 2023 Sep 21. doi: 10.1038/s41587-023-01951-0. 10.1038/s41587-023-01951-0 PubMed 37735263
