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Addgene

arginine biosensor cpFLIPR
(Plasmid #205746)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205746 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRSET-B
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 2711
  • Total vector size (bp) 5390
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    Room Temperature
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cpFLIPR
  • Alt name
    ECFP-cpAncQ-Venus
  • Alt name
    FRET-based biosensor for l-arginine
  • Species
    Synthetic
  • Insert Size (bp)
    2679
  • GenBank ID
    N/A
  • Tags / Fusion Proteins
    • 6 x Histidine purification tag (N-terminal)
    • Xpress™tag (N-terminal)
    • Biotin tag (N-terminal)
    • ECFP (N-terminal on cpAncQ binding protein)
    • Venus (C-terminal on cpAncQ binding protein)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS found the same amino acid sequence but 10 different codons within cpAncQ and a minor backbone deletion as compared to the depositor's supplemental sequence. The depositor has confirmed that this will not affect cpFLIPR function

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    arginine biosensor cpFLIPR was a gift from Colin Jackson (Addgene plasmid # 205746 ; http://n2t.net/addgene:205746 ; RRID:Addgene_205746)
  • For your References section:

    Construction of a robust and sensitive arginine biosensor through ancestral protein reconstruction. Whitfield JH, Zhang W, Herde MK, Clifton BE, Radziejewski J, Janovjak H, Henneberger C, Jackson CJ. Protein Sci. 2015 Jun 9. doi: 10.1002/pro.2721. 10.1002/pro.2721 PubMed 26061224