arginine biosensor cpFLIPR
(Plasmid
#205746)
-
PurposeRobust FRET biosensor enabling sensitive, accurate l-arginine measurement at physiological temp
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 205746 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepRSET-B
-
Backbone manufacturerAddgene
- Backbone size w/o insert (bp) 2711
- Total vector size (bp) 5390
-
Vector typeUnspecified
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth TemperatureRoom Temperature
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namecpFLIPR
-
Alt nameECFP-cpAncQ-Venus
-
Alt nameFRET-based biosensor for l-arginine
-
SpeciesSynthetic
-
Insert Size (bp)2679
-
GenBank IDN/A
-
Tags
/ Fusion Proteins
- 6 x Histidine purification tag (N-terminal)
- Xpress™tag (N-terminal)
- Biotin tag (N-terminal)
- ECFP (N-terminal on cpAncQ binding protein)
- Venus (C-terminal on cpAncQ binding protein)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene NGS found the same amino acid sequence but 10 different codons within cpAncQ and a minor backbone deletion as compared to the depositor's supplemental sequence. The depositor has confirmed that this will not affect cpFLIPR function
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
arginine biosensor cpFLIPR was a gift from Colin Jackson (Addgene plasmid # 205746 ; http://n2t.net/addgene:205746 ; RRID:Addgene_205746) -
For your References section:
Construction of a robust and sensitive arginine biosensor through ancestral protein reconstruction. Whitfield JH, Zhang W, Herde MK, Clifton BE, Radziejewski J, Janovjak H, Henneberger C, Jackson CJ. Protein Sci. 2015 Jun 9. doi: 10.1002/pro.2721. 10.1002/pro.2721 PubMed 26061224