arginine biosensor cpFLIPR
(Plasmid #205746)

• Purpose: Robust FRET biosensor enabling sensitive, accurate l-arginine measurement at physiological temp
• Depositing Lab: Colin Jackson
• Publication: Whitfield et al Protein Sci. 2015 Jun 9. doi: 10.1002/pro.2721.

Backbone

• Vector backbone: pRSET-B
• Backbone manufacturer: Addgene
• Backbone size w/o insert (bp): 2711
• Total vector size (bp): 5390
• Vector type: Unspecified

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: Room Temperature
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cpFLIPR
• Alt name: ECFP-cpAncQ-Venus
• Alt name: FRET-based biosensor for l-arginine
• Species: Synthetic
• Insert Size (bp): 2679
• GenBank ID: N/A
• Tags / Fusion Proteins:
  • 6 x Histidine purification tag (N-terminal)
  • Xpress™tag (N-terminal)
  • Biotin tag (N-terminal)
  • ECFP (N-terminal on cpAncQ binding protein)
  • Venus (C-terminal on cpAncQ binding protein)

Resource Information

• Supplemental Documents:
  • pDOTS10_cpAncQ (1) (3).gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene NGS found the same amino acid sequence but 10 different codons within cpAncQ and a minor backbone deletion as compared to the depositor's supplemental sequence. The depositor has confirmed that this will not affect cpFLIPR function