Lenti-BAT-RED
(Plasmid #20674)

• Depositing Lab: Tannishtha Reya
• Publication: Reya 2009 (unpublished)

Backbone

• Vector backbone: pBluescript-BATgal
• Backbone size w/o insert (bp): 7146
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Wnt reporter dsRED
• Tag / Fusion Protein:
  • NLS (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: M13for20
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

History:

1)From BAT-gal vector (S. Piccolo lab), cut with HindIII+BamHI to remove lacZ sequence including the nuclear sequence and the polyA sequence.

2) from DsRED.T4 (B. Glick lab), PCR-amplified DsRED + SV40 polyA with primers that contain the predicted NLS sequence from BATgal (see attachments)