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px-458-sgRNA-scramble
(Plasmid #206924)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206924 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    px458
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 9289
  • Total vector size (bp) 9292
  • Vector type
    CRISPR
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    In LB medium
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    non-targeting human sgRNA guides
  • gRNA/shRNA sequence
    non-targeting human sgRNA guides
  • Species
    H. sapiens (human)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)
  • 3′ cloning site BbsI (destroyed during cloning)
  • 5′ sequencing primer GACTATCATATGCTTACCGT
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    px-458-sgRNA-scramble was a gift from José Cuezva (Addgene plasmid # 206924 ; http://n2t.net/addgene:206924 ; RRID:Addgene_206924)

  • For your References section:

    IF1 promotes oligomeric assemblies of sluggish ATP synthase and outlines the heterogeneity of the mitochondrial membrane potential. Romero-Carraminana I, Esparza-Molto PB, Dominguez-Zorita S, Nuevo-Tapioles C, Cuezva JM. Commun Biol. 2023 Aug 12;6(1):836. doi: 10.1038/s42003-023-05214-1. 10.1038/s42003-023-05214-1 PubMed 37573449
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