pLPO4
(Plasmid #209978)

• Purpose: Contains Level 0 Part: replication origin (pWV01) for the construction of Level 1 plasmids. **Plasmid has been discontinued. See Comments section below for suggestions from depositing lab.
• Depositing Lab: Thorsten Mascher
• Publication: Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2779. doi: 10.1021/acssynbio.4c00075. Epub 2024 Sep 10.

Backbone

• Vector backbone: pLP3
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: replication origin (pWV01)
• Species: Lactobacilli

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsmBI (destroyed during cloning)
• 3′ cloning site: BsmBI (destroyed during cloning)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Note from depositing lab: The ori pWV01 in pLP04 was cloned from plasmid pTRK892 (Addgene #71803, https://www.addgene.org/71803/) We strongly suggest users who need this ori and experience growth or sequencing problems trying to clone this plasmid in their own lab either by buying plasmid pTRK892 from Addgene or synthesizing the ori as a DNA fragment and integrating it into our entry vector pLP3 (Addgene #209958 https://www.addgene.org/209958/)