pTwist GFP-Linker-VDAC1
(Plasmid
#211735)
-
PurposeExpresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the N-terminus of VDAC1 with a flexible linker.
-
Depositing Lab
-
Depositing OrganizationMedical University of Graz
Located in Austria -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 211735 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 211735-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 211735-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepTwist
-
Backbone manufacturerTwist Bioscience
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameVoltage-dependent anion channel 1
-
Insert Size (bp)1583
-
Entrez GeneVDAC1 (a.k.a. PORIN, VDAC-1)
- Promoter CMV
-
Tag
/ Fusion Protein
- GFP (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown
- (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.09.08.556841 for bioRxiv preprint.
DNA (Catalog # 211735-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 211735-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pTwist GFP-Linker-VDAC1 was a gift from Roland Malli (Addgene plasmid # 211735 ; http://n2t.net/addgene:211735 ; RRID:Addgene_211735) -
For your References section:
Visualizing VDAC1 in live cells using a tetracysteine tag. Pilic J, Oflaz FE, Gottschalk B, Erdogan YC, Graier WF, Malli R. PLoS One. 2024 Oct 18;19(10):e0311107. doi: 10.1371/journal.pone.0311107. eCollection 2024. 10.1371/journal.pone.0311107 PubMed 39423216
Map uploaded by the depositor.
